human nestin antibody  (Bio-Techne corporation)

Journal: Scientific Reports

Article Title: Impact of LIN7A silencing on U87 cell invasion and its clinical significance in glioblastoma

doi: 10.1038/s41598-025-91285-5

Figure Lengend Snippet: The detailed information of antibodies used in this study.

Article Snippet: Primary antibody , Anti-human nestin (rabbit polyclonal) , IF 1:200 , ZRB69 , MilliporeSigma.

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Transcriptional landscape of the interaction of human Mesenchymal Stem Cells with Glioblastoma in bioprinted co-cultures

doi: 10.1186/s13287-024-04022-6

Figure Lengend Snippet: Bioprinting of MSC and GBM primary cells: morphological aspects A ) Schematic illustration of bioprinting of scaffold. B ) Bioprinted scaffold with (a) confocal image of tumoroid structure in scaffold and (b) labeling of tumoroid with antibodies directed against GFAP (green), nestin (red) and nuclei (blue). C ) Use of transwell to analyze the migration of MSC toward GBM in the presence of defined medium (DM) or conditioned medium (CM) obtained from 72 h GBM cultures. Images were taken 4 h later. D ) Migration of MSC CAF towards tumor cells in scaffold, beginning of tumoroid formation in scaffolds

Article Snippet: For immunocytochemistry, biospheres were fixed with 4% paraformaldehyde for 1 h then permeabilized with 0.1% Triton X-100 for 30 min saturated with 5% BSA and then incubated with rabbit anti-human nestin (Proteintech, Rosemont, IL, USA) and mouse anti-human anti-GFAP (Proteintech).

Techniques: Labeling, Migration